CHE301 Audio Assignment No.1 Solution Fall 2019




CHE301 Audio Assignment No.1

separation techniques; a brief introduction:
As a part of sample preparation we have to separate our analyte in first phase. Our matrix may be gas, solid, liquid or semi solid.

Our analyte may be soluble or suspended in matrix. First we have to start with separation or semi purification. 

• Filtration • To separate solid from liquid • Sublimation • To separate solid from solids • 

Solvent Extraction • To separate solvents between two immiscible liquids • Solid Phase Extraction • 
To separate soluble substances from liquid samples .

Filtration 
• To separate solid from liquid 

• Sublimation 
• To separate solid from solids

• Solvent Extraction 
• To separate solvents between two immiscible liquids • Solid Phase Extraction

• To separate soluble substances from liquid samples

 • To separate solid from solid sublimation can be used as an analytical technique. 
Chromatographic techniques
 • Types of chromatography 

• Paper chromatography (1 and 2D)

 • Thin layer chromatography 

• Column chromatography 

• UV Spectroscopy 

• To find wavelength 

• To estimate concentration of unknown in sample 

• IR Spectroscopy 

• Use to evaluate functionalities in an Organic molecule 

• NMR spectroscopy 

 • On basis of magnetic properties define certain nuclei 

• There are different spectroscopic techniques available in market to solve our different analytical problems. 
The most widely used technique is UV Spectroscopy. 
That is basic instrument found in food and drug analysis laboratories.

technique for either finding out wavelength maximum of any molecule or use it for evaluation of concentration. N,N-dimethyl cinnamaldehyde .
We use this technique for either finding out wavelength maximum of any molecule or use it for evaluation of concentration. N,N

• UV Spectroscopy

• To find wavelength 

• To estimate concentration of unknown in sample 

• IR Spectroscopy 

• Use to evaluate functionalities in an Organic molecule 






CHE301 Audio Assignment No.1 Solution Fall 2019

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